Anti-mouse mesangial cell serum induces acute glomerulonephropathy in mice.
Nephron Exp Nephrol
; 93(3): e92-106, 2003.
Article
en En
| MEDLINE
| ID: mdl-12660412
ABSTRACT
In order to develop a model in mouse similar to anti- Thy-1 nephritis in the rat, we prepared sheep antiserum against SV40-transformed mouse mesangial (MES 13) cells. In vivo, the anti-mouse mesangial cell serum-treated mice showed severe azotemia that peaked at day 6 and proteinuria that peaked at day 8, in a dose-dependent fashion. Light microscopy and electron microscopy showed duplication of glomerular basement membranes, mesangiolysis, subendothelial and mesangial electron-dense deposits, and foot process effacement. Intraglomerular tuft cell number was significantly reduced at day 4 and there were increased numbers of apoptotic cells at days 2 and 4. SCID mice and mice lacking C3 manifested similar responses to anti-mouse mesangial cell serum, suggesting that T cells, B cells and complement are not required for glomerular injury in this model. In vitro, anti-mouse mesangial cell serum treated mesangial cells showed greater release of lactate dehydrogenase, decreased cell survival, and increased apoptotic cell death. Anti-mouse mesangial cell serum induces glomerulopathy characterized by mesangiolysis and mesangial cell apoptosis, and followed by cellular proliferation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glomerulonefritis Membranosa
/
Mesangio Glomerular
/
Sueros Inmunes
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Nephron Exp Nephrol
Asunto de la revista:
NEFROLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos