Photo-oxidation of duplex DNA with the stable trioxatriangulenium ion.
Bioorg Med Chem Lett
; 13(8): 1491-4, 2003 Apr 17.
Article
en En
| MEDLINE
| ID: mdl-12668019
ABSTRACT
We show that 5'-Gs in 5'-GG-3' duplex DNA dinucleotide steps are preferentially oxidized by the trioxatriangulenium ion (TOTA( plus sign in circle )) upon photo-activation, an oxidation pattern characteristic of guanine radical cation formation. Some photo-oxidation of the 3'G in 5'GG3' steps and of isolated guanines is also observed but reactions carried out in D(2)O reveal only a minor increase in oxidation damage at these sites, indicating that electron transfer is the primary mechanism of guanine oxidation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pirenos
/
ADN
/
Ácidos Nucleicos Heterodúplex
Idioma:
En
Revista:
Bioorg Med Chem Lett
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos