[Effects of arginine and growth hormone, administered in vivo and in vitro, on the oxygen consumption of rat liver hemogenates]. / Effets de l'arginine et de l'hormone croissance administrées "in vivo" et "in vitro" sur la consommation d'oxygène d'homogénats de foie de rat

ABSTRACT

Effects of arginine aspartate, arginine chlorhydrate, potassium aspartate, with equal arginine base and aspartate ion available for all there compounds, growth hormone, and association of these different substances, are studied on rat hepatic homogenates oxygen consumption during 7 to 12 h, after injection to rats 1 h before sacrifice (A.A. 0.50 g, C.A. 0.34 g, A.K. 0.22 g, STH 2 U.I.), or "in vitro" adjunction to the preparation (A.A. 0.125 g, C.A. 0.085 g. A.K. 0.055 g. STH 0.5 U.I./0.25 g of liver). 1) "In vivo", arginine (endogenous STH secretion stimulation) is active A.A. (+23% to +96% from 1 h to 8 h, p is less than 0.01), C.A. (+49% from 5 h to 8 h, p is less than 0.01), whereas potassium aspartate is inactive. "In vitro", aspartate is active A.K. (+24% to +147% from 4 h to 12 h, (p is less than 0.01), A.A. (+56% to +53% from 7 h to 12 h, p is less than 0.01), whereas arginine without aspartate C.A. is inactive. 2) Exogenous STH is active "in vivo" (+111% from 5 h to 8 h, p is less than 0.01) and "in vitro" (+31% from 4 h to 7 h, p is less than 0.01). 3) Association A.A. + STH (endogenous STH + exogenous STH) when given "in vivo", dose not produce any additive effect the tissue Vo2 release (+91% from 5 h to 8 h, p is less than 0.01) does not differ from STH and A.A. Aspartate added to STH "in vitro" enhances STH effects (A.A. + STH + 44% to +114% from 4 h to 12 h, p is less than 0.01, A.K. + STH 73% to + 152% from 4 h to 12 h, p is less than 0.01).