Used leucodepletion filters as a source of large quantities of DNA suitable for the study of genetic variations in human populations.
Transfus Med
; 13(2): 77-81, 2003 Apr.
Article
en En
| MEDLINE
| ID: mdl-12694552
ABSTRACT
UNLABELLED A simple technique for developing large control panels with large quantities of DNA suitable for studies in population genetics was established. BACKGROUND AND OBJECTIVES:
Both a lack of suitable controls and insufficient quantities of DNA for repeated analysis of the same control group often hamper the investigation of genetic markers for disease. MATERIALS ANDMETHODS:
Using a waste product from routine blood donation, we describe a simple method that allows the investigator to extract large amounts of DNA.RESULTS:
A mean of 1520 microg of DNA per sample was obtained. The DNA obtained remains suitable for polymerase chain reaction and sequencing techniques after 2 years of storage at both 4 degrees C and -40 degrees C.CONCLUSION:
This technique allows the development of a large panel of controls with sufficient quantities of genomic DNA for thousands of tests.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Variación Genética
/
ADN
/
Leucocitos
Límite:
Humans
Idioma:
En
Revista:
Transfus Med
Asunto de la revista:
HEMATOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Reino Unido