The 3alpha-hydroxy-steroid-dehydrogenase-mRNA and -protein are more prevalent in pericentral than in periportal hepatocytes.
Eur J Gastroenterol Hepatol
; 15(5): 509-13, 2003 May.
Article
en En
| MEDLINE
| ID: mdl-12702908
ABSTRACT
BACKGROUND:
Under physiological conditions, de-novo synthesis and metabolism of bile acids are confined mainly to the pericentral zone of the liver acinus. In the rat, 3alpha-hydroxy-steroid-dehydrogenase (3alpha-HSD) is the major bile acid-binding protein. At the same time, this protein is involved in the de-novo synthesis and metabolism of bile acids. Because bile acid processing is greater in the pericentral than in the periportal region, we investigated whether 3alpha-HSD is more prevalent in the pericentral than in the periportal area.DESIGN:
We determined the 3alpha-HSD-protein and its mRNA in periportal and pericentral rat cells.METHOD:
Rat hepatocytes from the periportal or pericentral areas were isolated using the digitonin perfusion technique. For Northern blotting, a labelled 1.3-kb cDNA insert corresponding to the mRNA sequence of 3alpha-HSD was used. For Western blotting, a polyclonal rabbit antiserum against human 3alpha-HSD was used. Blots were quantified by densitometry using phosphoimaging.RESULTS:
The amounts of the 3alpha-HSD-protein and its mRNA were significantly greater in the pericentral than in the periportal cells.CONCLUSIONS:
The greater occurrence of 3alpha-HSD in pericentral than in periportal hepatocytes is in line with the concept that bile acid synthesis and metabolism take place predominantly in pericentral cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Hepatocitos
/
3-Hidroxiesteroide Deshidrogenasas
Límite:
Animals
Idioma:
En
Revista:
Eur J Gastroenterol Hepatol
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Alemania