Genomic sequence of a 320-kb segment of the Z chromosome of Bombyx mori containing a kettin ortholog.
Mol Genet Genomics
; 269(1): 137-49, 2003 Apr.
Article
en En
| MEDLINE
| ID: mdl-12715162
ABSTRACT
The sex chromosome constitution of the silkworm, Bombyx mori, is ZW in the female and ZZ in the male. Very little molecular information is available about the Z chromosome in Lepidoptera, although the topic is interesting because of the absence of gene dosage compensation in this chromosome. We constructed a 320-kb BAC contig around the Bmkettin gene on the Z chromosome in Bombyx and determined its nucleotide sequence by the shotgun method. We found 13 novel protein-coding sequences in addition to Bmkettin. All the transposable elements detected in the region were truncated, and no LTR retrotransposons were found, in stark contrast to the situation on the W chromosome. In this 320-kb region, four genes for muscle proteins (Bmkettin, Bmtitin1, Bmtitin2, and Bmprojectin) are clustered, together with another gene (Bmmiple) on the Z chromosome in B. mori; their orthologs are also closely linked on chromosome 3 in Drosophila, suggesting a partial synteny. Real-time RT-PCR experiments demonstrated that transcripts of 13 genes of the 14 Z-linked genes found accumulated in larger amounts in males than in female moths, indicating the absence of gene dosage compensation. The implications of these findings for the evolution and function of the Z chromosome in Lepidoptera are discussed.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromosomas Sexuales
/
Bombyx
/
Genes de Insecto
/
Proteínas de Insectos
/
Proteínas de Drosophila
/
Proteínas Musculares
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Genet Genomics
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón