High diversity of non-human leukocyte antigens in transplant-associated coronary artery disease.
Transplantation
; 75(8): 1347-50, 2003 Apr 27.
Article
en En
| MEDLINE
| ID: mdl-12717228
ABSTRACT
BACKGROUND:
Antibodies to endothelial derived non-human leukocyte antigens (HLA) have been associated with transplant (Tx)-associated coronary artery disease (CAD) after cardiac transplantation; however, few have been identified. The aim of this study was to screen a human coronary artery endothelial cell cDNA library with patient sera to establish the diversity and nature of the target antigens.METHODS:
A human coronary artery endothelial cell cDNA library was screened with sera from seven long-term cardiac transplant patients with angiographically diagnosed TxCAD and sera from five healthy volunteers.RESULTS:
Of the seven patients' sera, five showed reactivity, as did sera from two of the five normal subjects. Eighteen positive cDNA clones were isolated by TxCAD sera; DNA sequence analysis and DNA database searching identified all but one clone; 16 were nuclear or cytoplasmic proteins and 1 of them was the cell surface protein neuropilin 2. Five clones were targeted by normal sera. A different spectrum of reactive clones was identified by the sera of each patient where reactive clones were evident.CONCLUSIONS:
A high diversity of non-HLA antigens, probably autoantigens, are involved in the pathogenesis of TxCAD.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trasplante de Corazón
/
Enfermedad Coronaria
/
Antígenos
Tipo de estudio:
Risk_factors_studies
Límite:
Adult
/
Humans
/
Middle aged
Idioma:
En
Revista:
Transplantation
Año:
2003
Tipo del documento:
Article
País de afiliación:
Reino Unido