Electrochemical detection of protein-protein interactions using a yeast two hybrid: 17-beta-estradiol as a model.
Anal Biochem
; 317(1): 34-9, 2003 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-12729598
ABSTRACT
In this work we present a modified yeast two-hybrid bioassay for the highly sensitive detection of protein-protein interactions, based on the electrochemical monitoring of beta-D-galactosidase reporter gene activity, using p-aminophenyl-beta-D-galactopyranoside (PAPG) as a synthetic substrate. In a model system, the sensitive detection of 17-beta-estradiol was achieved at concentrations as low as 10(-11)M (approx 2 pg/ml) by monitoring 17-beta-estradiol receptor dimerization after exposure to 17-beta-estradiol. The sensitivity of this system was higher than that of standard optical methods by three orders of magnitude.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Levaduras
/
Receptores de Estradiol
/
Técnicas del Sistema de Dos Híbridos
/
Estradiol
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Biochem
Año:
2003
Tipo del documento:
Article
País de afiliación:
Israel