Effect of reinsertion of activated nucleus pulposus on disc degeneration: an experimental study on various types of collagen in degenerative discs.
Connect Tissue Res
; 44(2): 104-8, 2003.
Article
en En
| MEDLINE
| ID: mdl-12745677
ABSTRACT
We examined the emergence and sequential changes in type I, II, and VI collagen production in an experimental rabbit model of disc degeneration. Type I collagen was minimally present initially and did not change over 24 weeks. Type I collagen seemed to have no effect on the degenerative process in this model. Staining for type II collagen was positive circumferentially in chondrocytelike cells and was mild in the early phase of disc degeneration, when the chondrocytelike cells began to appear in the inner layers of the annulus fibrosus. The stain became stronger during the middle phase when the chondrocytelike cells arranged themselves in cluster. Compared with type II collagen, the staining for type VI collagen was relatively strong early in the degenerative process. These findings led us to speculate that these chondrocytelike cells play an active role in the degenerative process. The reinsertion of nucleus pulposus cells cocultured with annulus fibrosus delayed disc degeneration and the emergence of chondrocytelike cells. Considering that the emergence of chondrocytelike cells which produce type II and type VI collagen is delayed in discs with the injection of cocultured nucleus pulposus cells by annulus fibrosus cells, we conclude that chondrocytelike cells that produce type VI collagen also seems to accelerate degeneration. Type VI collagen is produced at an earlier phase than type II collagen and may be both active agent and a marker for disc degeneration.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Colágeno
/
Tratamiento Basado en Trasplante de Células y Tejidos
/
Desplazamiento del Disco Intervertebral
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Connect Tissue Res
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón