Severe reduction of superoxide dismutase activity in the yeast Saccharomyces cerevisae with the deletion or overexpression of GTS1.
FEMS Microbiol Lett
; 223(1): 141-5, 2003 Jun 06.
Article
en En
| MEDLINE
| ID: mdl-12799013
ABSTRACT
We report herein that the level of reactive oxygen species (ROS) observed using dihydrorhodamine is much higher in either GTS1-deleted (gts1Delta) or GTS1-overexpressing (TMpGTS1) transformants than in the wild-type and that the levels of protein carbonyls are increased and the glutathione levels are decreased in both transformants. Consistently, the activities of superoxide dismutases (SODs) in both gts1Delta and TMpGTS1 were severely weakened, while the protein levels of both Cu/Zn-SOD and Mn-SOD were not so changed. As the intracellular copper levels were significantly increased in both transformants, we hypothesized that, in either gts1Delta or TMpGTS1 cells, the imbalanced homeostasis of copper induced an accumulation of ROS which caused inactivation of SODs further increasing ROS levels.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Superóxido Dismutasa
/
Factores de Transcripción
/
Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón