Growth dynamics of Artemisia annua hairy roots in three culture systems.
Biotechnol Bioeng
; 83(4): 428-43, 2003 Aug 20.
Article
en En
| MEDLINE
| ID: mdl-12800137
ABSTRACT
The transient growth of Artemisia annua hairy roots was compared for cultures grown in shake flasks and in bubble column and mist reactors. Instantaneous growth rates were obtained by numerically differentiating the transient biomass measurements. Specific sugar consumption rates showed good agreement with literature values. From the growth rate and sugar consumption rate, the specific yield and maintenance coefficient for sugar were determined for all three culture systems. These values were statistically indistinguishable for roots grown in shake flasks and bubble columns. In contrast, the values for roots grown in bubble columns and mist reactors were statistically different, suggesting that sugar utilization by roots grown in these two systems may be different. By measuring respiration rates in the bubble column reactor we also determined the actual biomass yield and maintenance coefficient for O(2) and CO(2). Together with an elemental analysis of the roots, this allowed us to obtain a reasonable carbon balance.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Raíces de Plantas
/
Reactores Biológicos
/
Artemisia annua
/
Técnicas de Cultivo
/
Metabolismo de los Hidratos de Carbono
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Idioma:
En
Revista:
Biotechnol Bioeng
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos