A method for extraction and analysis of high quality genomic DNA from ixodid ticks.
Med Vet Entomol
; 17(2): 224-7, 2003 Jun.
Article
en En
| MEDLINE
| ID: mdl-12823841
ABSTRACT
Currently, no published methods describe the extraction of high molecular weight genomic DNA from ixodid ticks (Acari Ixodidae) and commonly used methods of extraction are not well adapted for use with members of this family. A method for extraction of minimally degraded genomic DNA from ixodid ticks that can be completed in one or two days is described. The method produces DNA which is of sufficient size (>24 kb) for use in Southern analysis and which is readily digestible by restriction endonucleases. Southern analysis using a cytochrome P450 gene probe, demonstrates the success of our method with genomic DNA extracted from two species of Ixodidae, the lone star tick, Amblyomma americanum (Linnaeus) and the cattle fever tick, Boophilus microplus (Canestrini).
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Genoma
/
Ixodidae
Límite:
Animals
Idioma:
En
Revista:
Med Vet Entomol
Asunto de la revista:
BIOLOGIA
/
MEDICINA VETERINARIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos