Identification of tumor-associated antigens by screening phage-displayed human cDNA libraries with sera from tumor patients.
Int J Cancer
; 106(4): 534-544, 2003 Sep 10.
Article
en En
| MEDLINE
| ID: mdl-12845649
ABSTRACT
Screening cDNA libraries from solid human tumors with sera of autologous patients (SEREX) has proven to be a powerful approach to identifying tumor antigens recognized by the humoral arm of the immune system. In many cases, application of this methodology has led to the discovery of novel tumor antigens as unknown gene products. We tried to improve the potency of the SEREX approach by combining it with phage-display technology. We designed a new lambda vector to express protein fragments as N-terminal fusions to the D capsid protein and generated high-complexity cDNA libraries from human breast carcinoma cell lines and solid tumors. Screening these phage-displayed libraries required limited amounts of sera from patients and efficiently identified several tumor antigens specifically reacting with sera from breast cancer patients.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de la Mama
/
ADN de Neoplasias
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Biblioteca de Genes
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Antígenos de Neoplasias
Tipo de estudio:
Diagnostic_studies
/
Risk_factors_studies
/
Screening_studies
Límite:
Adult
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Aged
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Animals
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Int J Cancer
Año:
2003
Tipo del documento:
Article
País de afiliación:
Italia