Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif.
Cell
; 114(1): 21-31, 2003 Jul 11.
Article
en En
| MEDLINE
| ID: mdl-12859895
ABSTRACT
The HIV-1 accessory protein Vif (virion infectivity factor) is required for the production of infectious virions by CD4(+) lymphocytes. Vif facilitates particle infectivity by blocking the inhibitory activity of APOBEC3G (CEM15), a virion-encapsidated cellular protein that deaminates minus-strand reverse transcript cytosines to uracils. We report that HIV-1 Vif forms a complex with human APOBEC3G that prevents its virion encapsidation. HIV-1 Vif did not efficiently form a complex with mouse APOBEC3G. Vif dramatically reduced the amount of human APOBEC3G encapsidated in HIV-1 virions but did not prevent encapsidation of mouse or AGM APOBEC3G. As a result, these enzymes are potent inhibitors of wild-type HIV-1 replication. The species-specificity of this interaction may play a role in restricting HIV-1 infection to humans. Together these findings suggest that therapeutic intervention that either induced APOBEC3G or blocked its interaction with Vif could be clinically beneficial.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virión
/
Replicación Viral
/
Proteínas
/
Infecciones por VIH
/
Productos del Gen vif
/
VIH-1
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Cell
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos