A case of pulmonary aspergilloma molecular biological identification and typing of the isolates from antemortem sputa and autopsy fungus ball.

ABSTRACT

Filamentous fungi were isolated from antemortem sputum and an autopsy fungus ball of the lung in a case of aspergilloma. Both of the isolates were analyzed for the sequences of species or strain-specific nuclear ribosomal DNA (partial 28S and ITS1 regions), and were identified as Aspergillus fumigatus. The molecular biological technique saved time and is thought to be a powerful tool in the accurate diagnosis of pulmonary fungal infection to assure effective treatment.