[Overexpression of STAT3 in COS7 cells causes prominent morphological changes].
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 35(8): 717-22, 2003 Aug.
Article
en Zh
| MEDLINE
| ID: mdl-12897966
ABSTRACT
Signal transducer and activator of transcription 3 (STAT3) plays a central role in mediating signals related to cell growth, differentiation, survival and movement. In this report, the results of immunocytochemistry and Western blot analysis showed that the active form of Stat3 protein existed in COS7 cells. The endogenous Stat3 protein could also induce the expression of m67-sequence-directed reporter genes. The transient transfection of Stat3 cDNA into COS7 cells increased Stat3 protein level and the expression of the reporter gene. The overexpression of Stat3 protein in COS7 cells caused prominent cell morphological changes. These cells had a much larger cell body, extended long processes with branches, lamellipodia and filopodia. These results suggest that Stat3 protein may play important roles in cell adhesion, migration and cytoskeleton reorganization.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transactivadores
/
Tamaño de la Célula
/
Proteínas de Unión al ADN
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
Zh
Revista:
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
Año:
2003
Tipo del documento:
Article
País de afiliación:
China