Characteristics of collagenolytic enzymes secreted by deuteromycete fungi Aspergillus flavus.

Sukhosyrova, E A; Nikitina, Z K; Yakovleva, M B; Veshchikova, E V; Bykov, V A

Sukhosyrova, E A; Nikitina, Z K; Yakovleva, M B; Veshchikova, E V; Bykov, V A.

Afiliación

Sukhosyrova EA; Research and Training and Methodical Center for Biomedical Technologies VILAR, Moscow.

Bull Exp Biol Med ; 135(5): 447-51, 2003 May.

Article en En | MEDLINE | ID: mdl-12910283

RESUMEN

Collagen induced the synthesis of collagenolytic enzymes in the culture of Aspergillus flavus. Enzyme activity of the culture increased during storage and passage on a medium with this inductor. We developed a method for isolation and purification of collagenolytic enzymes and obtained two electrophoretically homogenous enzyme preparations belonging to neutral thermolabile collagenolytic metalloproteinases.

Asunto(s)

Aspergillus flavus/enzimología; Colágeno/metabolismo; Endopeptidasas/metabolismo; Aminoácidos/metabolismo; Técnicas de Cultivo de Célula/métodos; Quelantes/química; Medios de Cultivo/química; Ácido Edético/química; Endopeptidasas/aislamiento & purificación; Sacarosa/metabolismo

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Endopeptidasas / Aspergillus flavus / Colágeno Idioma: En Revista: Bull Exp Biol Med Año: 2003 Tipo del documento: Article Pais de publicación: Estados Unidos

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