Culture of human osteoblasts on demineralised human bone. Possible means of graft enhancement.
J Bone Joint Surg Br
; 74(2): 284-6, 1992 Mar.
Article
en En
| MEDLINE
| ID: mdl-1312095
ABSTRACT
We cultured human osteoblasts from trabecular bone explants and confirmed their phenotype by alkaline phosphatase assay, increased cyclic adenosine monophosphate production in response to prostaglandin E2 and radiographic micro-analysis of nodules of calcification. The osteoblasts were seeded on to demineralised human bone fragments and examined at ten-day intervals over a 50-day period by scanning electron microscopy. During this time the bank bone became progressively repopulated by the cultured osteoblasts. This system may offer a means of graft enhancement in elective orthopaedic and maxillofacial surgery by delivery of cultured autologous human osteoblasts to bone defects.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Huesos
/
Refuerzo Inmunológico de Injertos
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Bone Joint Surg Br
Año:
1992
Tipo del documento:
Article