SEC65 gene product is a subunit of the yeast signal recognition particle required for its integrity.
Nature
; 356(6369): 532-3, 1992 Apr 09.
Article
en En
| MEDLINE
| ID: mdl-1313947
ABSTRACT
Protein targeting to the endoplasmic reticulum (ER) in mammalian cells is catalysed by the signal recognition particle (SRP), which consists of six protein subunits and an RNA subunit. Saccharomyces cerevisiae SRP is a 16S particle, of which only two subunits have been identified a protein subunit, SRP54p, which is homologous to the mammalian SRP54 subunit, and an RNA subunit, scR1 (ref. 3). The sec65-1 mutant yeast cells are temperature-sensitive for growth and defective in the translocation of several secreted and membrane-bound proteins. The DNA sequence of the SEC65 gene suggests that its product is related to mammalian SRP19 subunit and may have a similar function. Here we show that SEC65p is a subunit of the S. cerevisiae SRP and that it is required for the stable association of another subunit, SRP54p, with SRP. Overexpression of SRP54p suppresses both growth and protein translocation defects in sec65-1 mutant cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ribonucleoproteínas
/
Saccharomyces cerevisiae
/
Proteínas Fúngicas
/
Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
Nature
Año:
1992
Tipo del documento:
Article