Half-lives of different sized mRNAs for the PKA subunit RI alpha are regulated differently in response to inhibition of transcription and translation.
Biochem Biophys Res Commun
; 184(1): 454-60, 1992 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-1314594
ABSTRACT
The RI alpha mRNA level is induced 3-5 times by FSH or cAMP analogs in primary cultures of rat Sertoli cells. In rat tissues, the RI alpha gene gives rise to three different mRNAs of different size 3.2, 2.9 and 1.7 kb. In the present study we report that the 1.7 kb transcript has a shorter half-life than the two other mRNAs. In cells which had been pre-stimulated with a cAMP analog, inhibition of transcription stabilizes the two larger, but not the smaller sized RI alpha mRNA. However, in contrast, inhibition of protein synthesis stabilizes all the RI alpha mRNAs. Thus, degradation of various mRNAs coding for the same protein reveals different dependencies on transcription and translation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Quinasas
/
Células de Sertoli
/
Transcripción Genética
/
Biosíntesis de Proteínas
/
ARN Mensajero
/
Regulación Enzimológica de la Expresión Génica
/
Bucladesina
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1992
Tipo del documento:
Article
País de afiliación:
Noruega