Immunocytochemical localization of estrogen receptors within neurotensin cells in the rostral preoptic area of the rat hypothalamus.
Neurosci Lett
; 136(1): 5-9, 1992 Feb 17.
Article
en En
| MEDLINE
| ID: mdl-1321967
ABSTRACT
In situ hybridization procedures indicate that estrogen selectively increases neurotensin and neuromedin (NT/N) mRNA levels in the rostral preoptic area of the rat hypothalamus (RPH). Using the co-localization procedures of Axelson and Van Leeuwen, J. Neuroendocrinol., 2 (1990) 209-216, the present study examined whether NT cells in the RPH contained estrogen receptors (ER). Vibratome sections of brains from adult ovariectomized, colchicine-treated rats were first incubated with estrogen receptor antibody and stained with diaminobenzidine (DAB)-Ni+ producing a blue-black nucleus. Subsequently, NT antisera were used to provide a brown reaction product with DAB as chromogen. Approximately 25% of the NT cells in the RPH contained ER. These data support the hypothesis that NT cells in the RPH that play a role in luteinizing hormone release from the pituitary are, in part, influenced directly by estrogen feedback via nuclear ER and may act as interneurons controlling luteinizing hormone releasing hormone turnover.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Área Preóptica
/
Neurotensina
/
Receptores de Estrógenos
Límite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Año:
1992
Tipo del documento:
Article
Pais de publicación:
IE
/
IRELAND
/
IRLANDA