Characterization, purification and serology of the Czechoslovak isolate of radish mosaic virus.
Acta Virol
; 36(2): 191-7, 1992 Mar.
Article
en En
| MEDLINE
| ID: mdl-1359771
ABSTRACT
The radish mosaic virus 1 (RMV 1) isolate was characterized according to the reaction of differential test plants and then purified by the chloroform-butanol method. UV-analysis of sucrose density gradients at 254 nm revealed three components. The values of A260/280 ratio were 1.42 for the top, 1.49 for the middle and 1.67 for the bottom component, respectively. Electron microscopy of the purified virus revealed isometric particles about 30 nm in diameter. Two antisera were prepared with the titre 11024 and 12048, respectively. The RMV 1 antigen formed a distinct precipitation line with the antiserum against the California neo-type strain of RMV.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus del Mosaico
Idioma:
En
Revista:
Acta Virol
Año:
1992
Tipo del documento:
Article