Characterization and nucleotide sequences of the variable regions of a monoclonal antibody against alpha-fetoprotein.
Hybridoma
; 11(3): 339-50, 1992 Jun.
Article
en En
| MEDLINE
| ID: mdl-1379974
ABSTRACT
alpha-Fetoprotein (AFP) is a well-known tumor marker of hepatocellular carcinoma (HCC). Monoclonal antibodies against AFP possessing specific binding ability to HCC are potential candidates for immunoscintigraphy and immunotherapy. A new monoclonal antibody against AFP (0325-6-9) was isolated. Its specificity and targeting tumor ability were characterized by enzyme-linked immunosorbent assay (ELISA), cell immunostain and complement killing. These results suggest that 0325-6-9 is specific to hepatoma cells. The nucleotide sequences of variable regions of 0325-6-9 were determined by M13 dideoxynucleotide sequencing method. With the information of nucleotide sequence, this antibody then could be modified by recombinant technology for its usage in in vivo diagnosis and immunotherapy.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Alfa-Fetoproteínas
/
Anticuerpos Monoclonales
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Hybridoma
Año:
1992
Tipo del documento:
Article