Cultured embryonic bone shafts show osteogenic responses to mechanical loading.
Calcif Tissue Int
; 51(2): 132-6, 1992 Aug.
Article
en En
| MEDLINE
| ID: mdl-1422952
ABSTRACT
Pairs of 17-day embryonic chick tibiotarsi were removed and maintained in organ culture. One of each pair was subjected to a single 20-minute period of intermittent loading at 0.4 Hz, producing peak longitudinal compressive strains of 650 microstrain (mu epsilon). In the 18-hour culture period following loading, alkaline phosphatase levels in the osteoblasts of the loaded tibiotarsi were maintained whereas in controls they declined. In situ hybridization using a collagen type I cRNA riboprobe showed a substantial increase in expression of mRNA for collagen type I in the periosteal tissue of bones that were cultured for 18 hours after loading compared with that in similarly cultured controls and bones cultured for 4 hours. These results demonstrate that appropriate loading of embryonic chick bones in organ culture elicits adaptive regulation of matrix synthesis as evidenced by increased expression of the gene for type I collagen and alkaline phosphatase activity. This model may be useful as it must contain all the obligatory steps between strain change in the matrix and modified osteogenic activity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Tarso Animal
/
Tibia
/
Colágeno
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Calcif Tissue Int
Año:
1992
Tipo del documento:
Article
País de afiliación:
Reino Unido