Nitrogen status modulates the expression of RNA-binding proteins in cyanobacteria.
FEMS Microbiol Lett
; 227(2): 203-10, 2003 Oct 24.
Article
en En
| MEDLINE
| ID: mdl-14592710
ABSTRACT
Biochemical responses to cold and osmotic stresses overlap because each decreases the availability of free water. Since RNA-binding proteins are known to accumulate following cold stress and play key roles in regulating transcription termination, the effect of osmotic stress on expression of RNA-binding proteins was examined. The transcript levels of four genes encoding RNA-binding proteins (rbpA, rbpB, rbpC and rbpD) were monitored in Anabaena sp. PCC 7120 cultures supplemented with ammonium ions or growing under nitrogen-fixing conditions. Steady-state transcript levels of all four genes increased transiently in response to a temperature shift from 30 to 20 degrees C under both nitrogen regimes. Osmotic stress also enhanced rbpB, rbpC and rbpD gene expression in ammonium grown cultures. In the absence of a combined nitrogen source, osmotic stress repressed the short-term induction of rbp gene expression. The accumulation of RNA-binding proteins did not follow transcript levels, but remained high 24 h after stress initiation. It is concluded that nitrogen nutrition modulates the stress-responsive regulation of RNA-binding proteins in cyanobacteria, providing a potential mechanism to integrate environmental and developmental signals.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcripción Genética
/
Regulación Bacteriana de la Expresión Génica
/
Proteínas de Unión al ARN
/
Anabaena
/
Frío
/
Nitrógeno
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM