Quantitative measurement of biotin-binding immunoglobulin G in human sera using F(ab')2 anti-human IgG-coated multi-well plates.
Biol Pharm Bull
; 26(11): 1605-8, 2003 Nov.
Article
en En
| MEDLINE
| ID: mdl-14600410
Biotin-binding human immunoglobulin G (B-IgG) was quantitatively measured using an F(ab')2anti-human IgG-coated multi-well microplate for the first time. B-IgG was caught by F(ab')2anti-human IgG and was detected by the following detecting reagents: Peroxidase-labeled streptavidin, avidin and peroxidase-biotin, or avidin-biotinylated peroxidase complex with method A, B, or C, respectively. Commercially available B-IgG was detected by all these three methods. However, method A and B could not detect B-IgG in the human sera used in this study, but we quantitatively measured the B-IgG level using method C. The result is probably due to the fact that the sensitivity of method C was higher than that of methods A or B. Properties of B-IgG detected by method C are discussed in the text.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sitios de Unión de Anticuerpos
/
Biotina
/
Inmunoglobulina G
/
Receptores Inmunológicos
Límite:
Humans
Idioma:
En
Revista:
Biol Pharm Bull
Asunto de la revista:
BIOQUIMICA
/
FARMACOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Japón