A silkworm larvae plasma test for detecting peptidoglycan in cerebrospinal fluid is useful for the diagnosis of bacterial meningitis.
Microbiol Immunol
; 47(10): 701-7, 2003.
Article
en En
| MEDLINE
| ID: mdl-14605436
ABSTRACT
The silkworm larvae plasma (SLP) test has been established based on a cascade reaction triggered by either peptidoglycan or (1, 3)-beta-D-glucan to produce melanin. We applied this test to the diagnosis of bacterial meningitis. Cerebrospinal fluid (CSF) obtained from patients with bacterial meningitis due to gram-positive bacteria, gram-negative bacteria, or fungi, showed positive reactions to the test. In contrast, CSF from patients with viral meningitis or noninfectious illnesses gave negative reactions. Therefore, this test seems to be useful for diagnosis of bacterial and fungal meningitis. When this test was used together with two types of limulus tests, an endotoxin-specific test, and a conventional test, meningitis was further characterized as gram-positive, gram-negative or fungal meningitis. The SLP test requires a computerized instrument for quantitative colorimetric measurement. A qualitative alternative of this test also can be accomplished by visually observing the darkening color. Thus, this method can be applied for simple and rapid diagnosis of meningitis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bombyx
/
Peptidoglicano
/
Líquido Cefalorraquídeo
/
Meningitis Bacterianas
/
Bacterias Gramnegativas
/
Bacterias Grampositivas
Tipo de estudio:
Diagnostic_studies
/
Qualitative_research
Límite:
Adolescent
/
Adult
/
Animals
/
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
/
Newborn
Idioma:
En
Revista:
Microbiol Immunol
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón