Identification of the high-virulence clone of group B streptococci by using a probe containing a putative aldolase gene.
Curr Microbiol
; 47(4): 319-22, 2003 Oct.
Article
en En
| MEDLINE
| ID: mdl-14640070
ABSTRACT
A high-virulence clone (HVC) was proposed as causing much of the morbidity and mortality when a collection of group B Streptococcus (GBS) isolates was examined by multi-locus enzyme electrophoresis. HVC isolates could be further distinguished by their inability to grow at 40 degrees C, and a temperature-sensitive aldolase was identified as responsible for this characteristic. In the present study, the HVC was sought in a collection of 57 GBS isolates by hybridization with a probe containing a putative aldolase gene on genomic DNA restriction enzyme digests. Isolates were initially classified as HVC or non-HVC by their inability to grow at 40 degrees C. Three serotype III invasive isolates had the HVC control restriction/hybridization pattern. They were also unable to grow at 40 degrees C. The remaining 11 invasive and all carrier isolates showed a pattern identical to that of the non-HVC control. These results provide additional support for the existence of a highly virulent clonal group among serotype III isolates and suggest that hybridization with a probe containing the aldolase gene on DNA restriction enzyme digests can be an alternative method for identifying highly virulent isolates.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Infecciones Estreptocócicas
/
Streptococcus agalactiae
/
Sondas de ADN
/
Técnicas de Tipificación Bacteriana
/
Fructosa-Bifosfato Aldolasa
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Curr Microbiol
Año:
2003
Tipo del documento:
Article