Production of heterologous and chimeric scaffoldins by Clostridium acetobutylicum ATCC 824.
J Bacteriol
; 186(1): 253-7, 2004 Jan.
Article
en En
| MEDLINE
| ID: mdl-14679247
ABSTRACT
Clostridium acetobutylicum ATCC 824 converts sugars and various polysaccharides into acids and solvents. This bacterium, however, is unable to utilize cellulosic substrates, since it is able to secrete very small amounts of cellulosomes. To promote the utilization of crystalline cellulose, the strategy we chose aims at producing heterologous minicellulosomes, containing two different cellulases bound to a miniscaffoldin, in C. acetobutylicum. A first step toward this goal describes the production of miniCipC1, a truncated form of CipC from Clostridium cellulolyticum, and the hybrid scaffoldin Scaf 3, which bears an additional cohesin domain derived from CipA from Clostridium thermocellum. Both proteins were correctly matured and secreted in the medium, and their various domains were found to be functional.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Proteínas Recombinantes de Fusión
/
Proteínas Recombinantes
/
Celulosa
/
Clostridium
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
J Bacteriol
Año:
2004
Tipo del documento:
Article
País de afiliación:
Francia