Molecular assessment of ammonia- and nitrite-oxidizing bacteria in full-scale activated sludge wastewater treatment plants.
Water Sci Technol
; 48(8): 119-26, 2003.
Article
en En
| MEDLINE
| ID: mdl-14682578
ABSTRACT
Nitrification was assessed in two full-scale wastewater treatment plants (WWTPs) over time using molecular methods. Both WWTPs employed a complete-mix suspended growth, aerobic activated sludge process (with biomass recycle) for combined carbon and nitrogen treatment. However, one facility treated primarily municipal wastewater while the other only industrial wastewater. Real time PCR assays were developed to determine copy numbers for total 16S rDNA (a measure of biomass content), the amoA gene (a measure of ammonia-oxidizers), and the Nitrospira 16S rDNA gene (a measure of nitrite-oxidizers) in mixed liquor samples. In both the municipal and industrial WWTP samples, total 16S rDNA values were approximately 2-9 x 10(13) copies/L and Nitrospira 16S rDNA values were 2-4 x 10(10) copies/L. amoA gene concentrations averaged 1.73 x 10(9) copies/L (municipal) and 1.06 x 10(10) copies/L (industrial), however, assays for two distinct ammonia oxidizing bacteria were required.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aguas del Alcantarillado
/
Eliminación de Residuos Líquidos
/
Nitrógeno
Idioma:
En
Revista:
Water Sci Technol
Asunto de la revista:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos