Zinc binding by retroviral integrase.
Biochem Biophys Res Commun
; 189(2): 813-8, 1992 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-1472053
ABSTRACT
Zinc binding by integrase from Moloney murine leukaemia virus and a protein A fusion protein containing integrase from human immunodeficiency virus type 1 was demonstrated by a zinc blotting technique using 65ZnCl2. Autoradiography revealed a clear band that was absent from the appropriate controls. This band co-migrated with the major band in Coomassie-stained gels and in immunoblots. This binding activity was retained in the presence of competing divalent cations and was sensitive to oxidation. This is the first demonstration of zinc binding by intact retroviral integrase.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Zinc
/
VIH-1
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ADN Nucleotidiltransferasas
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Virus de la Leucemia Murina de Moloney
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1992
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
EEUU
/
ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
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USA