Development of a new Sindbis virus transducing system and its characterization in three Culicine mosquitoes and two Lepidopteran species.
Insect Mol Biol
; 13(1): 89-100, 2004 Feb.
Article
en En
| MEDLINE
| ID: mdl-14728670
ABSTRACT
Alphavirus transducing systems (ATSs) are alphavirus-based tools for expressing genes in insects. Here we describe an ATS (5'dsMRE16ic) based entirely on Sindbis MRE16 virus. GFP expression was used to characterize alimentary tract infections and dissemination in three Culicine and two Lepidopteran species. Following per os infection, 5'dsMRE16ic-EGFP efficiently infected Aedes aegypti and Culex tritaeniorhynchus, but not Culex pipiens pipiens. Ae. aegypti clearly showed accumulation of green fluorescent protein (GFP) in the posterior midgut and foregut/midgut junction within 2-3 days postinfection. Following parenteral infection of larvae, Bombyx mori had extensive GFP expression in larvae and adults, but Manduca sexta larvae were mostly resistant. 5'dsMRE16ic should be a valuable tool for gene expression in several important insect species that are otherwise difficult to manipulate genetically.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus Sindbis
/
Transducción Genética
/
Expresión Génica
/
Culicidae
/
Mariposas Nocturnas
Límite:
Animals
Idioma:
En
Revista:
Insect Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos