Harvesting airway surface liquid: a comparison of two techniques.
Pediatr Pulmonol
; 37(2): 149-57, 2004 Feb.
Article
en En
| MEDLINE
| ID: mdl-14730660
ABSTRACT
The quantity and composition of airway surface liquid (ASL) are essential to host defense. To date, attempts to harvest ASL and measure its composition have yielded conflicting results. We investigated the physical principles underlying two techniques that were proposed for harvesting ASL filter paper pledgets and polyethylene catheters. We compared the force and pressure generation and the kinematics of capillarity-induced fluid uptake with both techniques. Both have significant limitations for harvesting ASL, generating physiologically significant pressures (filter paper, 60.4 Pa; polyethylene, 14.3 Pa) that could potentially compromise epithelial integrity. Furthermore, filter paper generates a force 85-fold higher than the polyethylene catheter, which is associated with a very high rate of uptake of liquid and a large total amount of liquid relative to ASL thickness. While the PE catheter harvests liquid more gently, it is only effective when ASL surface tension is below 31 mN/m. These limitations likely account for some of the variability in reported ASL composition, and highlight the need for improved methods for harvesting ASL.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Líquidos Corporales
/
Electroforesis Capilar
/
Mucosa Respiratoria
Límite:
Humans
Idioma:
En
Revista:
Pediatr Pulmonol
Asunto de la revista:
PEDIATRIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Canadá