Purification of biologically active human plasma transthyretin by dye-affinity chromatography: studies on dye leakage and possibility of heat treatment for virus inactivation.
J Chromatogr
; 584(1): 93-9, 1992 Dec 11.
Article
en En
| MEDLINE
| ID: mdl-1487520
ABSTRACT
The application of a purification procedure for the industrial preparation from human plasma of a therapeutic protein may be hindered by several safety concerns. The dye leaching from Remazol Yellow GGL-Sepharose used for the affinity chromatography of human plasma transthyretin was quantitatively studied by a sensitive competitive enzyme immunoassay. The possibility of including a heat treatment step for virus inactivation in the purification process while preserving the biochemical and functional characteristics of the protein is also reported.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Azo
/
Prealbúmina
/
Cromatografía de Afinidad
/
Fenómenos Fisiológicos de los Virus
/
Calor
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr
Año:
1992
Tipo del documento:
Article