Construction of a shuttle lacZ alpha-based Escherichia coli-actinomycetes vector containing the phage JHJ-3 replicon.
FEMS Microbiol Lett
; 78(2-3): 257-60, 1992 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-1490607
ABSTRACT
Using the broad replicating range JHJ-3 phage replicon, a shuttle vector for Escherichia coli and actinomycetes has been constructed. The vector, pOJ31, bears the lacZ alpha fragment allowing a blue/white gene cloning system. pOJ31 also contains a polylinker of 15 unique cloning sites and the phage T7 promoter. The vector has been used to stably express the mel gene from plasmid pIJ702 in Streptomyces lividans.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Replicón
/
Actinomycetales
/
Escherichia coli
/
Vectores Genéticos
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
1992
Tipo del documento:
Article
País de afiliación:
Canadá