An immunohistochemical study of mesothelial cell seeding for knitted Dacron.
Int J Exp Pathol
; 73(6): 801-8, 1992 Dec.
Article
en En
| MEDLINE
| ID: mdl-1493108
ABSTRACT
Six greyhounds underwent bilateral femoral artery replacement with knitted Dacron, one side seeded with omental digest at graft preclotting, the other acting as an unseeded control. Grafts were removed at 24 hours and two months. Tissue was examined using a monoclonal antibody (MNF116) directed against a broad range of human cytokeratins to differentiate mesothelial cells (MC) from microvascular endothelial cells (MEC), which stained only with a polyclonal antibody directed against von Willebrand Factor (anti-vWF). Cells released from omentum by collagenase stained with MNF116 and reacted poorly with anti-vWF. Identical cells were observed to be within the interstices of seeded but not control knitted Dacron. Few remained in seeded grafts (n = 2) removed at 24 hours and none at two months (n = 4).
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Epiplón
/
Prótesis Vascular
/
Arteria Femoral
Límite:
Animals
Idioma:
En
Revista:
Int J Exp Pathol
Asunto de la revista:
PATOLOGIA
Año:
1992
Tipo del documento:
Article
País de afiliación:
Reino Unido