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Characterization of citrus HSVd isolates.
Palacio-Bielsa, A; Romero-Durbán, J; Duran-Vila, N.
Afiliación
  • Palacio-Bielsa A; Departamento de Protección Vegetal y Biotecnología, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Spain.
Arch Virol ; 149(3): 537-52, 2004 Mar.
Article en En | MEDLINE | ID: mdl-14991442
Seven citrus isolates of Hop stunt viroid (HSVd) were subjected to retrotranscription and DNA amplification (RT-PCR), cloning and sequencing. Single stranded polymorphism (SSCP) analysis demonstrated the existence of variability among and within cachexia inducing sources of HSVd. The electrophoretic profiles of SSCP appeared to be able to discriminate between non-cachexia and cachexia sources of HSVd. Sequence analysis demonstrated that the variable (V) domain was very conserved among the cachexia variants. Five nucleotide differences, affecting both the upper (3 nucleotides) and the lower (2 nucleotides) strands of the V domain, were identified as a motif discriminating cachexia and non-cachexia sequences. These five nucleotides affect the organization of a short helical region and two flanking loops of the V domain probably modifying the three-dimensional geometry of the molecule. The stability of the minimum free energy rod-like conformation of the cachexia sequences is lower than the non-cachexia. Information regarding the host effect on the evolution and variability of viroid quasispecies is also provided.
Asunto(s)
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Viroides / Citrus Tipo de estudio: Prognostic_studies Idioma: En Revista: Arch Virol Año: 2004 Tipo del documento: Article País de afiliación: España Pais de publicación: Austria
Buscar en Google
Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Viroides / Citrus Tipo de estudio: Prognostic_studies Idioma: En Revista: Arch Virol Año: 2004 Tipo del documento: Article País de afiliación: España Pais de publicación: Austria