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SCAR and CAPS mapping of CRb, a gene conferring resistance to Plasmodiophora brassicae in Chinese cabbage ( Brassica rapa ssp. pekinensis).
Piao, Z Y; Deng, Y Q; Choi, S R; Park, Y J; Lim, Y P.
Afiliación
  • Piao ZY; Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, 305-764 Daejeon, Korea.
Theor Appl Genet ; 108(8): 1458-65, 2004 May.
Article en En | MEDLINE | ID: mdl-14997298
Clubroot disease, caused by Plasmodiophora brassicae Wor., is highly damaging for Chinese cabbage. The CR (clubroot resistant) Shinki DH (doubled haploid) line of Chinese cabbage carries a single dominant gene, CRb, which confers resistance to the P. brassicae races 2, 4, and 8. An F(2) population derived from a cross between the CR Shinki DH line and a susceptible line, 94SK, was used to map the CRb gene. Inoculation of F(3) families with SSI (single-spore isolate) resulted in a 1:2:1 segregation ratio. Use of the AFLP technique combined with bulked segregant analysis allowed five co-dominant AFLP markers, and four and seven dominant AFLP markers linked in coupling and repulsion, respectively, to be identified. Six of the 16 AFLP markers showing low frequencies of recombination with the CRb locus among 138 F(2) lines were cloned. A reliable conversion procedure allowed five AFLP markers to be successfully converted into CAPS and SCAR markers. An F(2) population (143 plants) was analyzed with these markers and a previously identified SCAR marker, and a genetic map around CRb covering a total distance of 6.75 cM was constructed. One dominant marker, TCR09, was located 0.78 cM from CRb. The remaining markers (TCR05, TCR01, TCR10, TCR08, and TCR03) were located on the other side of CRb, and the nearest of these was TCR05, at a distance of 1.92 cM.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Brassica / Genes de Plantas / Hongos Idioma: En Revista: Theor Appl Genet Año: 2004 Tipo del documento: Article Pais de publicación: Alemania
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Brassica / Genes de Plantas / Hongos Idioma: En Revista: Theor Appl Genet Año: 2004 Tipo del documento: Article Pais de publicación: Alemania