Participation of transcription factors from the Rel/NF-kappa B family in the circadian system in hamsters.
Neurosci Lett
; 358(1): 9-12, 2004 Mar 18.
Article
en En
| MEDLINE
| ID: mdl-15016422
ABSTRACT
We have studied the presence and activity of components of the nuclear factor-kappaB (NF-kappaB) transcription factor in the hamster circadian system analyzing wheel-running activity, protein expression and DNA binding activity by electrophoresis mobility shift assays (EMSA). Non-rhythmic specific immunoreactive bands corresponding to a NF-kappaB subunit (p65) were found in hamster suprachiasmatic nuclei (SCN) homogenates. The active form of NF-kappaB evidenced by EMSA was clear and specific in SCN nuclear extracts. The administration of the NF-kappaB inhibitor pyrrolidine-dithiocharbamate (PDTC) blocked the light-induced phase advance at circadian time 18 (vehicle+light pulse 2.08+/-0.46 h, PDTC+light 0.36+/-0.35 h). These results demonstrate the presence and activity of Rel/NF-kappaB family proteins in the hamster SCN and suggest that these proteins may be related to the entrainment and regulation of circadian rhythms.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Núcleo Supraquiasmático
/
Factores de Transcripción
/
FN-kappa B
/
Ritmo Circadiano
/
Proteínas Oncogénicas v-rel
Límite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Año:
2004
Tipo del documento:
Article
País de afiliación:
Argentina