Co-immunoprecipitation from transfected cells.
Methods Mol Biol
; 261: 337-50, 2004.
Article
en En
| MEDLINE
| ID: mdl-15064468
ABSTRACT
One of the most commonly used methods for determining whether two proteins can interact is co-immunoprecipitation. Co-immunoprecipitation relies on the ability of an antibody to stably and specifically bind complexes containing a bait protein. The antibody provides a means of immobilizing these complexes on a solid matrix, which in the protocol presented here is accomplished through interaction with Protein A, so that irrelevant proteins can be washed away. The presence of target proteins in the bait complexes is determined by Western blot. Because of the biochemical diversity of protein-protein interactions, it is not possible to describe a single set of conditions that will work for every immunoprecipitation experiment. Instead, the goal of this chapter is to provide practical starting conditions for co-immunoprecipitation assays and to describe potential modifications to the procedure so that conditions can be optimized.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pruebas de Precipitina
/
Transfección
/
Proteínas
/
Mapeo de Interacción de Proteínas
Tipo de estudio:
Guideline
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos