Reconstitution of nicotinic acetylcholine receptors into gel-protected lipid membranes.
Anal Chem
; 76(8): 2261-5, 2004 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-15080736
ABSTRACT
We report the functional reconstitution of nicotinic acetylcholine receptors into gel-protected bilayer lipid membranes using two different methods. In the first case, reconstitution was achieved by direct membrane formation from an emulsion of glycerol monooleate, hexane, and a membrane receptor extract. In the second case, incorporation was achieved via the fusion of vesicles from a preparation of membrane-bound receptors into preformed membranes after diffusion through the protective front gel layer. Measurement of the dc conductivity of the membranes in the presence of either acetylcholine or alpha-bungarotoxin was used to test for the functional activity of incorporated receptors.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores Nicotínicos
/
Membrana Dobles de Lípidos
/
Lípidos de la Membrana
/
Membranas Artificiales
Idioma:
En
Revista:
Anal Chem
Año:
2004
Tipo del documento:
Article
País de afiliación:
Reino Unido