The effect of desferrioxamine on peroxynitrite-induced oxidative damage in erythrocytes.
Cell Biochem Funct
; 22(3): 149-52, 2004.
Article
en En
| MEDLINE
| ID: mdl-15124178
ABSTRACT
The aim of this study was to investigate the effect of desferrioxamine on peroxynitrite-mediated damage in erythrocytes by measuring the 3-nitrotyrosine level and glutathione peroxidase and Na(+)-K(+) ATPase activities in vitro. 3-Nitrotyrosine levels were determined by HPLC; glutathione peroxidase and Na(+)-K(+) ATPase activities were measured by spectrophotometry. Peroxynitrite increased the 3-nitrotyrosine level but decreased both enzyme activities. In the presence of desferrioxamine, glutathione peroxidase activity was increased with a decrease in the 3-nitrotyrosine level. Desferrioxamine was found to possess an important antioxidant activity as assessed in an in vitro system, reducing protein nitration, restoring enzyme activities and maintaining erythrocyte membrane integrity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tirosina
/
Estrés Oxidativo
/
Ácido Peroxinitroso
/
Deferoxamina
/
Eritrocitos
Límite:
Humans
Idioma:
En
Revista:
Cell Biochem Funct
Año:
2004
Tipo del documento:
Article
País de afiliación:
Turquía