Evaluation of developmental phenotypes produced by morpholino antisense targeting of a sea urchin Runx gene.
BMC Biol
; 2: 6, 2004 May 07.
Article
en En
| MEDLINE
| ID: mdl-15132741
ABSTRACT
BACKGROUND:
Runx transcription factors are important regulators of metazoan development. The sea urchin Runx gene SpRunt was previously identified as a trans-activator of the CyIIIa actin gene, a differentiation marker of larval aboral ectoderm. Here we extend the functional analysis of SpRunt, using morpholino antisense oligonucleotides (morpholinos) to interfere with SpRunt expression in the embryo.RESULTS:
The developmental effects of four different SpRunt-specific morpholinos were evaluated. The two morpholinos most effective at knocking down SpRunt produce an identical mitotic catastrophe phenotype at late cleavage stage that is an artifact of coincidental mis-targeting to histone mRNA, providing a cautionary example of the insufficiency of two different morpholinos as a control for specificity. The other two morpholinos produce gastrula stage proliferation and differentiation defects that are rescued by exogenous SpRunt mRNA. The expression of 22 genes involved in cell proliferation and differentiation was analyzed in the latter embryos by quantitative polymerase chain reaction. Knockdown of SpRunt was found to perturb the expression of differentiation markers in all of the major tissue territories as well as the expression of cell cycle control genes, including cyclin B and cyclin D.CONCLUSIONS:
SpRunt is essential for embryonic development, and is required globally to coordinate cell proliferation and differentiation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Erizos de Mar
/
Factores de Transcripción
/
Oligonucleótidos Antisentido
/
Regulación del Desarrollo de la Expresión Génica
/
Embrión no Mamífero
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
BMC Biol
Asunto de la revista:
BIOLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Estados Unidos