[Effects of substance P on granulation tissue fibroblasts proliferation and expression of basic fibroblast growth factor mRNA].
Zhonghua Wai Ke Za Zhi
; 42(6): 366-8, 2004 Mar 22.
Article
en Zh
| MEDLINE
| ID: mdl-15144694
ABSTRACT
OBJECTIVE:
To explore the proliferation-promoting effect of sensory neuropeptide substance P (SP) on the cultured granulation tissue fibroblasts in vitro and its regulative effect on the gene expression of basic fibroblast growth factor (bFGF) mRNA.METHODS:
The proliferation-promoting effect of cultured granulation tissue fibroblasts was observed by means of MTT; the regulative effect of SP on gene expression of fibroblast bFGF by RT-PCR. The time and dose-efficiency relations were also observed.RESULTS:
There was a significant proliferation-promoting effect of SP on the cultured granulation tissue fibroblasts in vitro in a remarkable dose-dependent fashion. However, bFGF antibody only partly exerted its inhibitive effect. SP could induce the bFGF mRNA expression of the fibroblasts at the 3rd and 6th hour (P < 0.01). SP could promote the bFGF mRNA expression of the fibroblasts in the concentration of 10(-9) - 10(-5) mol/L and peaked in the concentration of 10(-7) mol/L.CONCLUSIONS:
SP has a significant proliferation-promoting effect on the granulation tissue fibroblasts, which is correlated with SP inducing bFGF mRNA expression of fibroblasts.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sustancia P
/
Factor 2 de Crecimiento de Fibroblastos
/
Fibroblastos
/
Tejido de Granulación
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua Wai Ke Za Zhi
Año:
2004
Tipo del documento:
Article
País de afiliación:
China