[Screening anti-adhesion polypeptides from phage peptide library].

ABSTRACT

AIM: To screen the active peptides which can suppress adhesion of monocytes to endothelial cells(ECs) from a random peptide phage library. METHODS: The ECs injured by oxidized low density lipoprotein (ox-LDL) 100 mg/L was used as target cells and polypeptides specifically binding to the injured ECs were isolated from the phage-displayed peptide library. The positive clones were characterized by ELISA, and the amino acid sequences of the peptides were deduced by DNA sequencing. Cell counting was used to evaluate the adhesion rate between ECs and monocytes. RESULTS: Six positive clones specifically binding to injured EC were isolated. Four of them contained a repeated sequence of leucine-leucine. Among the four clones, two had an identical sequence, which could reduce the adhesion rate of monocytes to ECs by 17%. CONCLUSION: One peptide which can suppress adhesion of monocytes to ECs is isolated from a random peptide phage library.