ZEN-4/MKLP1 is required to polarize the foregut epithelium.
Curr Biol
; 14(11): 932-41, 2004 Jun 08.
Article
en En
| MEDLINE
| ID: mdl-15182666
ABSTRACT
BACKGROUND:
Epithelial tubes are a key component of organs and are generated from cells with distinct apico-basolateral polarity. Here, we describe a novel function during tubulogenesis for ZEN-4, the Caenorhabditis elegans ortholog of mitotic kinesin-like protein 1 (MKLP1), and CYK-4, which contains a RhoGAP (GTPase-activating protein) domain. Previous studies revealed that these proteins comprise centralspindlin (a complex that functions during mitosis to bundle microtubules), construct the spindle midzone, and complete cytokinesis.RESULTS:
Our analyses demonstrate that ZEN-4/MKLP1 functions postmitotically to establish the foregut epithelium. Mutants that lack ZEN-4/MKLP1 express polarity markers but fail to target these proteins appropriately to the cell cortex. Affected proteins include PAR-3/Bazooka and PKC-3/atypical protein kinase C at the apical membrane domain, and HMR-1/cadherin and AJM-1 within C. elegans apical junctions (CeAJ). Microtubules and actin are disorganized in zen-4 mutants compared to the wild-type.CONCLUSION:
We suggest that ZEN-4/MKLP1 and CYK-4/RhoGAP regulate an early step in epithelial polarization that is required to establish the apical domain and CeAJ.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cinesinas
/
Polaridad Celular
/
Proteínas Activadoras de GTPasa
/
Proteínas de Caenorhabditis elegans
/
Microtúbulos
/
Modelos Biológicos
Límite:
Animals
Idioma:
En
Revista:
Curr Biol
Asunto de la revista:
BIOLOGIA
Año:
2004
Tipo del documento:
Article