[Genetic diversity of Fujian local duck populations in different ecological type].

ABSTRACT

A total of 40 short primers of arbitrary nucleotide sequence were used singly in polymerase chain reaction to amplify DNA fingerprints in pools of DNA representing seven duck populations from two ecological areas in Fujian Province. 9 primers which discriminated between the breed-specific DNA pools were used further to amplify individual pool components. Shannon index was used to calculate the genetic diversity and its partition within and between the seven duck populations. The relationships between seven duck populations and ecological environment, and their variation patterns were studied by RAPD. The results showed that the genetic diversity in east Fujian (67.97%) was higher than that in west Fujian (59.05%). Genetic differentiation was estimated to be about 32.03% among populations of east Fujian, and about 40.95% among populations of west Fujian. The genetic distance index matrix and UPMGA dendrogram indicated that Liancheng white duck, Taining duck, Longyan duck and Sanming duck in west Fujian had closer genetic relationships, and Longhai Jinding duck and Putian black duck in east Fujian had the similarly. There were some evidences showing that relevance existed between the variation and the geographical position.