Nerve growth factor-induced substance P in capsaicin-insensitive vagal neurons innervating the lower mouse airway.
Clin Exp Allergy
; 34(9): 1474-9, 2004 Sep.
Article
en En
| MEDLINE
| ID: mdl-15347383
ABSTRACT
BACKGROUND:
Nerve growth factor (NGF) is elevated in allergic diseases such as bronchial asthma and can lead to an induction of substance P (SP) and related neuropeptides in guinea-pigs large-diameter, neurofilament-positive airway neurons.OBJECTIVE:
In the present study, the effect of NGF on tyrosine kinase receptor trkA and the capsaicin receptor TRPV1 expression in airway-specific vagal sensory neurons located in the jugular-nodose ganglia complex (JNC) of mice was investigated.METHODS:
Using retrograde neuronal tracing in combination with double-labelling immunohistochemistry, SP, trkA- and TRPV1-receptor expression was examined in airway-specific sensory neurons of BALB/c mice before and after NGF treatment.RESULTS:
NGF injected into the lower airway was able to induce SP (13.0+/-2.03% vs. 5.9+/-0.33%) and trkA expression (78+/-2.66% vs. 60+/-2.11%) in larger diameter (>25 microm), capsaicin-insensitive and trkA-positive vagal sensory neurons that were retrograde-labelled with Fast Blue dye from the main stem bronchi.CONCLUSION:
Based on the extent of SP and trkA co-expression in airway-specific neurons by NGF treatment, the present study suggests that, following a peripheral activation of trkA receptor on SP afferent by NGF which is elevated in allergic inflammation, there may be trkA-mediated SP induction to mediate neurogenic airway inflammation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sistema Respiratorio
/
Capsaicina
/
Sustancia P
/
Factor de Crecimiento Nervioso
/
Neuronas Aferentes
/
Ganglio Nudoso
Límite:
Animals
Idioma:
En
Revista:
Clin Exp Allergy
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Alemania