Analysis of fluorescent MRI contrast agent behavior in the liver and thoracic aorta of mice.
Anal Quant Cytol Histol
; 26(4): 233-8, 2004 Aug.
Article
en En
| MEDLINE
| ID: mdl-15457677
ABSTRACT
OBJECTIVE:
To characterize the behavior of magnetofluorescent products injected in mice intravenously. STUDYDESIGN:
The magnetic resonance imaging (MRI) products were labelled with fluorescent molecules to examine the biodistribution process in vivo and observe them at the cellular level by means of confocal microscopy. Three-dimensional (3D) sequences of images were obtained by spectral analysis of sample preparations in a multiphoton confocal microscope and analyzed by the factor analysis of medical image sequence algorithm, which provides factor curves. Factor images are the result of image-processing methods that utilize information from emission spectra. Preparations are also screened in the counting mode to provide fluorescent lifetime imaging microscopy (FLIM) characterizations.RESULTS:
Factor images and FLIM images can help to analyze MRI targeting inside the liver and thoracic aorta of mice. They show positive detection of Fe-Texas red and BOPTA-Eu in the liver and positive detection of Fe-Texas red and negative detection of BOPTA-Eu inside the thoracic aorta.CONCLUSION:
This investigation established the utility of fluorescent MRI contrast agents as in vivo staining tools for cellular sites.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Organometálicos
/
Aorta Torácica
/
Coloración y Etiquetado
/
Medios de Contraste
/
Hígado
/
Meglumina
Límite:
Animals
Idioma:
En
Revista:
Anal Quant Cytol Histol
Asunto de la revista:
HISTOLOGIA
Año:
2004
Tipo del documento:
Article
País de afiliación:
Francia