Improved capillary electrophoretic separation of nine (fluoro)quinolones with fluorescence detection for biological and environmental samples.
J Chromatogr A
; 1047(2): 305-11, 2004 Aug 27.
Article
en En
| MEDLINE
| ID: mdl-15460263
ABSTRACT
A capillary electrophoretic method has been developed for the separation of nine (fluoro)quinolones. Detection is done by fluorescence measurement with broad wavelength band excitation between 240 and 400 nm. Best separation is achieved in a carrier electrolyte containing 50 mM H3PO4 adjusted to pH 7.55-acetonitrile (6040, v/v). Detection limits are in the low microgl(-1) range. The suitability to real samples has been demonstrated by analyzing blood samples and surface water samples. Sample preconcentration and sample clean-up can easily be done by solid-phase extraction. Different phases based on alkyl- or phenyl-modified silica as well as on polymers have been investigated for this purpose. The method should also be useful for determination of residues of (fluoro)quinolones in food or other matrices.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
/
Electroforesis Capilar
/
Fluoroquinolonas
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
J Chromatogr A
Año:
2004
Tipo del documento:
Article
País de afiliación:
Austria